Production of Odd-Carbon Dicarboxylic Acids in Escherichia coli Using an Engineered Biotin-Fatty Acid Biosynthetic Pathway.

Autor:	Haushalter RW; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Phelan RM; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Hoh KM; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Su C; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Wang G; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Baidoo EE; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States., Keasling JD; Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.; Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2017 Apr 05; Vol. 139 (13), pp. 4615-4618. Date of Electronic Publication: 2017 Mar 21.
DOI:	10.1021/jacs.6b11895
Abstrakt:	Dicarboxylic acids are commodity chemicals used in the production of plastics, polyesters, nylons, fragrances, and medications. Bio-based routes to dicarboxylic acids are gaining attention due to environmental concerns about petroleum-based production of these compounds. Some industrial applications require dicarboxylic acids with specific carbon chain lengths, including odd-carbon species. Biosynthetic pathways involving cytochrome P450-catalyzed oxidation of fatty acids in yeast and bacteria have been reported, but these systems produce almost exclusively even-carbon species. Here we report a novel pathway to odd-carbon dicarboxylic acids directly from glucose in Escherichia coli by employing an engineered pathway combining enzymes from biotin and fatty acid synthesis. Optimization of the pathway will lead to industrial strains for the production of valuable odd-carbon diacids.
Databáze:	MEDLINE
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