Candida albicans Mycofilms Support Staphylococcus aureus Colonization and Enhances Miconazole Resistance in Dual-Species Interactions.

Autor:	Kean R; Oral Sciences Research Group, Glasgow Dental School - School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of GlasgowGlasgow, UK; Institute of Healthcare Policy and Practise - Institute of Healthcare Associated Infection, University of the West of ScotlandPaisley, UK., Rajendran R; Oral Sciences Research Group, Glasgow Dental School - School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow Glasgow, UK., Haggarty J; Glasgow Polyomics, University of Glasgow Glasgow, UK., Townsend EM; Oral Sciences Research Group, Glasgow Dental School - School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of GlasgowGlasgow, UK; Institute of Healthcare Policy and Practise - Institute of Healthcare Associated Infection, University of the West of ScotlandPaisley, UK., Short B; Oral Sciences Research Group, Glasgow Dental School - School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of GlasgowGlasgow, UK; Department of Life Sciences, School of Health and Life Sciences, Glasgow Caledonian UniversityGlasgow, UK., Burgess KE; Glasgow Polyomics, University of Glasgow Glasgow, UK., Lang S; Department of Life Sciences, School of Health and Life Sciences, Glasgow Caledonian University Glasgow, UK., Millington O; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde Glasgow, UK., Mackay WG; Institute of Healthcare Policy and Practise - Institute of Healthcare Associated Infection, University of the West of Scotland Paisley, UK., Williams C; Institute of Healthcare Policy and Practise - Institute of Healthcare Associated Infection, University of the West of Scotland Paisley, UK., Ramage G; Oral Sciences Research Group, Glasgow Dental School - School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow Glasgow, UK.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2017 Feb 23; Vol. 8, pp. 258. Date of Electronic Publication: 2017 Feb 23 (Print Publication: 2017).
DOI:	10.3389/fmicb.2017.00258
Abstrakt:	Polymicrobial inter-kingdom biofilm infections represent a clinical management conundrum. The presence of co-isolation of bacteria and fungi complicates the ability to routinely administer single antimicrobial regimens, and synergy between the microorganisms influences infection severity. We therefore investigated the nosocomial pathogens Staphylococcus aureus and Candida albicans with respect to antimicrobial intervention. We characterized the interaction using biofilm assays and evaluated the effect of miconazole treatment using in vitro and in vivo assays. Finally, we assessed the impact of biofilm extracellular matrix (ECM) on these interactions. Data indicated that the C. albicans mycofilms supported adhesion and colonization by S. aureus through close interactions with hyphal elements, significantly increasing S. aureus biofilm formation throughout biofilm maturation. Miconazole sensitivity was shown to be reduced in both mono- and dual-species biofilms compared to planktonic cells. Within a three-dimensional biofilm model sensitivity was also hindered. Galleria mellonella survival analysis showed both enhanced pathogenicity of the dual-species infection, which was concomitantly desensitized to miconazole treatment. Analysis of the ECM revealed the importance of extracellular DNA, which supported the adhesion of S. aureus and the development of the dual-species biofilm structures. Collectively, these data highlight the clinical importance of dual-species inter-kingdom biofilm infections, though also provides translational opportunities to manage them more effectively.
Databáze:	MEDLINE
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