Bioprospection of cold-adapted yeasts with biotechnological potential from Antarctica.

Autor: Martorell MM; Instituto Antártico Argentino (IAA), CONICET, Buenos Aires, Argentina., Ruberto LAM; Instituto Antártico Argentino (IAA), CONICET, Buenos Aires, Argentina.; Universidad de Buenos Aires, Buenos Aires, Argentina.; Instituto de Nanobiotecnología (NANOBIOTEC-UBA-CONICET), Buenos Aires, Argentina., Fernández PM; Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), San Miguel de Tucumán, Tucumán, Argentina., Castellanos de Figueroa LI; Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), San Miguel de Tucumán, Tucumán, Argentina.; Universidad Nacional de Tucumán (UNT), Tucumán, Argentina., Mac Cormack WP; Instituto Antártico Argentino (IAA), CONICET, Buenos Aires, Argentina.; Universidad de Buenos Aires, Buenos Aires, Argentina.; Instituto de Nanobiotecnología (NANOBIOTEC-UBA-CONICET), Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Journal of basic microbiology [J Basic Microbiol] 2017 Jun; Vol. 57 (6), pp. 504-516. Date of Electronic Publication: 2017 Mar 08.
DOI: 10.1002/jobm.201700021
Abstrakt: The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia. Results from enzymes production at low temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which represent potential tools for biotechnological applications. While most the isolates proved to produce 2-4 of the investigated exoenzymes, two of them evidenced the six evaluated enzymatic activities: Pichia caribbica and Guehomyces pullulans, which were characterized as psycrotolerant and psycrophilic, respectively. In addition, P. caribbica could assimilate several n-alkanes and diesel fuel. The enzyme production profile and hydrocarbons assimilation capacity, combined with its high level of biomass production and the extended exponential growth phase make P. caribbica a promising tool for cold environments biotechnological purposes in the field of cold-enzymes production and oil spills bioremediation as well.
(© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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