Transplantation of fecal microbiota from patients with irritable bowel syndrome alters gut function and behavior in recipient mice.

Autor:	De Palma G; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Lynch MD; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada., Lu J; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Dang VT; Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario, Canada., Deng Y; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Jury J; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Umeh G; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Miranda PM; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Pigrau Pastor M; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Sidani S; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Pinto-Sanchez MI; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Philip V; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., McLean PG; Nestlé Research Center, Lausanne, Switzerland., Hagelsieb MG; Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada., Surette MG; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Bergonzelli GE; Nestlé Research Center, Lausanne, Switzerland., Verdu EF; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Britz-McKibbin P; Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Ontario, Canada., Neufeld JD; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada., Collins SM; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada., Bercik P; Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada. bercikp@mcmaster.ca.
Jazyk:	angličtina
Zdroj:	Science translational medicine [Sci Transl Med] 2017 Mar 01; Vol. 9 (379).
DOI:	10.1126/scitranslmed.aaf6397
Abstrakt:	Irritable bowel syndrome (IBS) is a common disorder characterized by altered gut function and often is accompanied by comorbid anxiety. Although changes in the gut microbiota have been documented, their relevance to the clinical expression of IBS is unknown. To evaluate a functional role for commensal gut bacteria in IBS, we colonized germ-free mice with the fecal microbiota from healthy control individuals or IBS patients with diarrhea (IBS-D), with or without anxiety, and monitored gut function and behavior in the transplanted mice. Microbiota profiles in recipient mice clustered according to the microbiota profiles of the human donors. Mice receiving the IBS-D fecal microbiota showed a taxonomically similar microbial composition to that of mice receiving the healthy control fecal microbiota. However, IBS-D mice showed different serum metabolomic profiles. Mice receiving the IBS-D fecal microbiota, but not the healthy control fecal microbiota, exhibited faster gastrointestinal transit, intestinal barrier dysfunction, innate immune activation, and anxiety-like behavior. These results indicate the potential of the gut microbiota to contribute to both intestinal and behavioral manifestations of IBS-D and suggest the potential value of microbiota-directed therapies in IBS patients. (Copyright © 2017, American Association for the Advancement of Science.)
Databáze:	MEDLINE
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