| Autor: |
Glybochko PV; Sechenov First Moscow State Medical University., Alyaev YG; Department of Urology, Faculty of Medicine., Shekhter AB; Research Institute for Molecular Medicine., Vinarov AZ; Research Institute for Uronephrology and Reproductive Health., Istranov LP; Research Institute for Molecular Medicine., Istranova EV; Research Institute for Molecular Medicine., Aboyants RK; Research Institute for Molecular Medicine., Lyundup AV; Research Institute for Molecular Medicine., Krasheninnikov ME; Research Institute for Molecular Medicine., Guller AE; Research Institute for Molecular Medicine., Butnaru DV; Research Institute for Uronephrology and Reproductive Health., Marisov LV; Research Institute for Uronephrology and Reproductive Health., Kantimerov DF; Sechenov First Moscow State Medical University., Kurkov AV; Research Institute for Uronephrology and Reproductive Health., Safronova EI; Sechenov First Moscow State Medical University., Vorobeva EA; Sechenov First Moscow State Medical University., Iritsyan MM; Sechenov First Moscow State Medical University. |
| Abstrakt: |
Urethral strictures are a pressing issue in modern medicine. Substitution urethroplasty is considered one of the most effective treatment methods. However, despite the surgery showing good results, many problems remain unresolved, one being substitute material deficiency in extensive or recurrent strictures, as well as in cases requiring multistage surgeries, including those used to treat hypospadias. Graft removal also leaves the donor area prone to diseases and increases the length of surgery leading to a higher risk of intra- and postoperative complications. Tissue engineering (namely tissue-engineered products comprised of scaffolds and cells) may be a useful tool in dealing with these issues. The authors assessed the characteristics of a novel hybrid scaffold created from "reconstructed" collagen and a poly(lactic-co-glycolic acid) mesh. The resulting composite product showed good mechanical properties and functional performance. The hybrid scaffold was non-cytotoxic and provided an adequate base for cell adhesion and proliferation. Biodegradation resulted in the scaffold being replaced by urothelium and urethral mucosa. The newly formed tissues possessed adequate structural and functional properties. Only one rabbit out of 12 developed urethral stricture at the site of scaffold implantation. The above-mentioned facts suggest that the novel hybrid scaffold is a promising tissue-engineered product with potential implication in substitution urethroplasty. |
