Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.

Autor:	Mitzelfelt KA; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA., McDermott-Roe C; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: mcdec@mail.med.upenn.edu., Grzybowski MN; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Marquez M; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Kuo CT; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Riedel M; PharmaCell, 6229 Maastricht, the Netherlands., Lai S; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Choi MJ; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Kolander KD; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Helbling D; Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Dimmock DP; Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Battle MA; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Jou CJ; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA., Tristani-Firouzi M; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA., Verbsky JW; Section of Quantitative Health Sciences, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Benjamin IJ; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Geurts AM; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: ageurts@mcw.edu.
Jazyk:	angličtina
Zdroj:	Stem cell reports [Stem Cell Reports] 2017 Mar 14; Vol. 8 (3), pp. 491-499. Date of Electronic Publication: 2017 Feb 24.
DOI:	10.1016/j.stemcr.2017.01.021
Abstrakt:	Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models. (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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