Validation of commercial ELISAs for quantifying anabolic growth factors and cytokines in canine ACD-A anticoagulated plasma.

Autor: Birdwhistell K; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA., Basinger R; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA., Hayes B; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA., Norton N; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA., Hurley DJ; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA., Franklin SP; Department of Small Animal Medicine and Surgery (Birdwhistell, Basinger, Hayes, Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Regenerative Bioscience Center (Franklin), University of Georgia, College of Veterinary Medicine, Athens, GA.; Large Animal Medicine and Surgery (Norton, Hurley), University of Georgia, College of Veterinary Medicine, Athens, GA.
Jazyk: angličtina
Zdroj: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2017 Mar; Vol. 29 (2), pp. 143-147. Date of Electronic Publication: 2017 Feb 08.
DOI: 10.1177/1040638717690186
Abstrakt: Platelet-rich plasma has been studied extensively in dogs, but validation of enzyme-linked immunosorbent assays (ELISAs) for quantifying anabolic growth factors and inflammatory cytokines in canine plasma prepared with citrate-based anticoagulants is not available. We performed a validation of commercial ELISAs for transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) for use with canine plasma prepared with acid-citrate-dextrose, solution A (ACD-A). Platelet-poor plasma (PPP) anticoagulated with ACD-A as well as PPP anticoagulated with ACD-A and spiked with the relevant canine recombinant proteins were evaluated with each ELISA to calculate the efficiency of spike recovery. Replicates of the spiked PPP were also assessed in 2 additional assays to quantify intra-assay and interassay precision. The efficiency of spike recovery was within 75-125% of the expected concentration for the TGF-β1, PDGF-BB, and VEGF ELISAs. The intra- and interassay variability were <25% for the TGF-β1, PDGF-BB, VEGF, and TNF-α ELISAs. The TGF-β1, PDGF-BB, and VEGF ELISAs demonstrate acceptable efficiency of spike recovery and intra- and interassay variability, whereas the TNF-α and IL-1β ELISAs did not meet industry standards of performance with ACD-A anticoagulated canine plasma.
Databáze: MEDLINE
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