Antigen-specific secretion of IFNγ and CXCL10 in whole blood assay detects Mycobacterium leprae infection but does not discriminate asymptomatic infection from symptomatic leprosy.

Autor: Hungria EM; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-020, Brazil., Freitas AA; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-020, Brazil., Pontes MA; Centro de Dermatologia Dona Libânia, Fortaleza, CE 60035-100, Brazil., Gonçalves HS; Centro de Dermatologia Dona Libânia, Fortaleza, CE 60035-100, Brazil., Sousa AL; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-020, Brazil., Costa MB; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-020, Brazil., Castilho ML; Hospital de Doenças Tropicais/Dr Anuar Auad, Goiânia, GO 74605-020, Brazil., Duthie MS; Infectious Disease Research Institute, Seattle, WA 98102, USA., Stefani MM; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-020, Brazil. Electronic address: mmastefani@gmail.com.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Apr; Vol. 87 (4), pp. 328-334. Date of Electronic Publication: 2017 Jan 05.
DOI: 10.1016/j.diagmicrobio.2017.01.002
Abstrakt: To advance toward a whole blood assay (WBA)-based test capable of facilitating the diagnosis of paucibacillary (PB) leprosy, we evaluated a prototype in-tube WBA using combinations of Mycobacterium leprae antigens. Blood was collected from newly diagnosed untreated PB (n=38), multibacillary (MB) (n=30), healthy household contacts (HHC) of MB (n=27), and endemic controls (n=61) residing in Goiânia and Fortaleza, Brazil. Blood was incubated with M. leprae cell sonicate, recombinant proteins (46f+LID-1; ML0276+LID-1), or controls (phosphate-buffered saline, phytohemagglutinin, M. tuberculosis purified protein derivative). Antigen-specific IFNγ production was observed in 71-84% and 55% of PB and HHC, respectively. Antigen-specific CXCL10 levels were similarly assessed to determine if, unlike IFNγ, CXCL10 could differentiate PB from HHC with repeated exposure/asymptomatic M. leprae infection. The CXCL10 levels induced in response to M. leprae antigens could not, however, differentiate PB from HHC. Despite these limitations, the WBAs reported here still represent important tools for assessing M. leprae infection rates and evaluating the impact of control measures.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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