Silver nanoparticles induce hormesis in A549 human epithelial cells.

Autor: Sthijns MM; Department of Pharmacology and Toxicology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. Electronic address: mireille.sthijns@maastrichtuniversity.nl., Thongkam W; IUF - Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 40225 DE Düsseldorf, Germany., Albrecht C; IUF - Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 40225 DE Düsseldorf, Germany., Hellack B; Institute of Energy and Environmental Technology e.V. (IUTA), Bliersheimerstraße 58-60, 47229 Duisburg, Germany., Bast A; Department of Pharmacology and Toxicology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands., Haenen GR; Department of Pharmacology and Toxicology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands., Schins RP; IUF - Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 40225 DE Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2017 Apr; Vol. 40, pp. 223-233. Date of Electronic Publication: 2017 Jan 18.
DOI: 10.1016/j.tiv.2017.01.010
Abstrakt: Despite the gaps in our knowledge on the toxicity of silver nanoparticles (AgNPs), the application of these materials is fast expanding, from medicine, to food as well as the use in consumer products. It has been reported that prolonged exposure might make cells more resistant to AgNPs. This prompted us to investigate if AgNPs may give rise to a hormetic response. Two types of AgNPs were used, i.e. colloidal AgNPs and an AgNP powder. For both types of nanosilver it was found that a low dose pretreatment of A549 human epithelial cells with AgNPs induced protection against a toxic dose of AgNPs and acrolein. This protection was more pronounced after pretreatment with the colloidal AgNPs. Interestingly, the mechanism of the hormetic response appeared to differ from that of acrolein. Adaptation to acrolein is related to Nrf2 translocation, increased mRNA expression of γGCS, HO-1 and increased GSH levels and the increased GSH levels can explain the hormetic effect. The adaptive response to AgNPs was not related to an increase in mRNA expression of γGCS and GSH levels. Yet, HO-1 mRNA expression and Nrf2 immunoreactivity were enhanced, indicating that these processes might be involved. So, AgNPs induce adaptation, but in contrast to acrolein GSH plays no role.
(Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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