| Autor: |
El-Badawy MF; 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology , Cairo, Egypt .; 2 Department of Pharmaceutical Microbiology, College of Pharmacy, Taif University , Taif, Saudi Arabia ., Tawakol WM; 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology , Cairo, Egypt ., Maghrabi IA; 3 Department of Clinical Pharmacy, College of Pharmacy, Taif University , Taif, Saudi Arabia ., Mansy MS; 4 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University , Cairo, Egypt ., Shohayeb MM; 2 Department of Pharmaceutical Microbiology, College of Pharmacy, Taif University , Taif, Saudi Arabia .; 5 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University , Tanta, Egypt ., Ashour MS; 4 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University , Cairo, Egypt . |
| Abstrakt: |
The extensive use of β-lactam antibiotics has led to emergence and spread of extended-spectrum β-lactamases (ESBLs). This study was conducted to investigate the prevalence of 7 different ESBL genes (bla TEM , bla SHV , bla CTX-M , bla VEB , bla PER , bla GES , and bla OXA-10 ) and O25b-ST131 high-risk clone among 61 clinical isolates of Escherichia coli. Also, one broad-spectrum β-lactamase (bla OXA-1 ) was investigated. This study was also constructed to evaluate iodometric overlay method in detection of ESBL production. Phenotypic identification of E. coli isolates using API 20E revealed 18 distinct biotypes. DNA fingerprinting using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) differentiated all isolates into 2 main phylogenetic groups with 60 distinct genetic profiles. Elevated values of minimal inhibitory concentration (MIC) 50 and MIC 90 for third- and fourth-generation cephalosporins were observed. Phenotypic tests revealed that 85.24% of isolates were ESBL producers. The incidence rates of bla TEM , bla SHV , bla CTX-M , bla GES , bla OXA-1 , and bla OXA-10 among E. coli ESBL producer phenotype were 69.23%, 25%, 96.15%, 3.85%, 11.54%, and 48%, respectively. On the other hand, bla VEB and bla PER were not detected. Sequencing of bla TEM and bla SHV revealed that bla TEM-214 and bla SHV-11 were the most prevalent variants. Group characterization of bla CTX-M revealed that bla CTX-M-1 was the most prevalent group of bla CTX-M family. It was found that 30.77% of E. coli ESBL producers belonged to O25b-ST131 clone harboring bla CTX-M-15 . This study concluded that iodometric overlay method was 100% sensitive in detection of ESBL production. To our knowledge, this is the first Egyptian study that declares the emergence of E. coli O25b-ST131 harboring bla GES . |
