Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.

Autor: Patil SS; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Prajapati A; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Hemadri D; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Suresh KP; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Desai GS; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Reddy GB; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India., Chandranaik BM; Institute of Animal Health and Veterinary Biologicals, Bengaluru - 560 024, Karnataka, India., Ranganatha S; Institute of Animal Health and Veterinary Biologicals, Bengaluru - 560 024, Karnataka, India., Rahman H; Division of Animal Sciences, Indian Council of Agriculture Research, Krishi Bhawan, New Delhi - 110 001, India.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2016 Dec; Vol. 9 (12), pp. 1364-1369. Date of Electronic Publication: 2016 Dec 05.
DOI: 10.14202/vetworld.2016.1364-1369
Abstrakt: Aim: This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease.
Materials and Methods: Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software.
Results: A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain.
Conclusion: BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.
Databáze: MEDLINE
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