Anti-diarrhoeal therapeutic potential and safety assessment of sulphated polysaccharide fraction from Gracilaria intermedia seaweed in mice.

Autor: Leódido AC; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Costa LE; Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60455-760, Brazil., Araújo TS; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Costa DS; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Sousa NA; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Souza LK; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Sousa FB; Biotechnology and Biodiversity Center Research, BIOTEC, Federal University of Piauí - CMRV, Parnaíba, PI 64202-020, Brazil., Filho MD; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Vasconcelos DF; Post-graduation Program in Biomedical Sciences, Federal University of Piauí, Parnaíba, PI 64202-020, Brazil., Silva FR; Post-graduation Program in Biomedical Sciences, Federal University of Piauí, Parnaíba, PI 64202-020, Brazil., Nogueira KM; Biotechnology and Biodiversity Center Research, BIOTEC, Federal University of Piauí - CMRV, Parnaíba, PI 64202-020, Brazil., Araújo AR; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil., Barros FC; Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60455-760, Brazil; Federal Institute of Education, Science and Technology of Ceará, Juazeiro do Norte, Ceará 63040-540, Brazil., Freitas AL; Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60455-760, Brazil., Medeiros JV; The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil; Biotechnology and Biodiversity Center Research, BIOTEC, Federal University of Piauí - CMRV, Parnaíba, PI 64202-020, Brazil; Post-graduation Program in Biomedical Sciences, Federal University of Piauí, Parnaíba, PI 64202-020, Brazil. Electronic address: jandvenes@ufpi.edu.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2017 Apr; Vol. 97, pp. 34-45. Date of Electronic Publication: 2017 Jan 05.
DOI: 10.1016/j.ijbiomac.2017.01.006
Abstrakt: Sulphated polysaccharides extracted from algae have been extensively studied for their diverse biological activities. Thus, the purpose of this study was to evaluate the chemical composition, the anti-diarrhoeal effect and acute toxicity of a sulphated polysaccharide fraction obtained from Gracilaria intermedia (SP-Gi). Initially, the FT-IR of SP-Gi revealed to be an agaran with sulphation at C-6 of the l-galactosyl residues. The anti-diarrhoeal activity of SP-Gi was evaluated in a castor oil-induced diarrhoea model. The effects of SP-Gi on enteropooling, Na + -K + -ATPase activity, gastrointestinal transit, and gastric emptying were then examined. Subsequently, the effect of SP-Gi on diarrhoea induced by cholera toxin (CT) and Escherichia coli was examined. In addition, an acute toxicity test was conducted in accordance with OECD guideline 423. Pre-treatment with SP-Gi reduces the total faeces, total diarrhoeal faeces, and enteropooling. SP-Gi (30mg/kg p.o.) increased Na + /K + -ATPase activity and reduced gastrointestinal transit through anticholinergic mechanisms. ELISA demonstrated that SP-Gi can interact with GM1 receptors and CT. SP-Gi reduced diarrhoea induced by E. coli and prevented weight loss in the animals. Moreover, SP-Gi did not induce any toxicity signs. These results suggest that SP-Gi is a possible candidate for the treatment of diarrhoeal illnesses.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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