Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules.

Autor:	Duboc C; Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France., Fan J; Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France., Graves ET; Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France., Strick TR; Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France; Ecole Normale Supérieure, Institut de Biologie de l'ENS (iBENS), INSERM, CNRS, PSL Research University, Paris, France. Electronic address: strick@ijm.univ-paris-diderot.fr.
Jazyk:	angličtina
Zdroj:	Methods in enzymology [Methods Enzymol] 2017; Vol. 582, pp. 275-296. Date of Electronic Publication: 2016 Dec 03.
DOI:	10.1016/bs.mie.2016.09.048
Abstrakt:	Simultaneous nanomanipulation and colocalization of single molecules (NanoCOSM) provides a unique opportunity to correlate the mechanical properties and activities of biomolecules with their conformational states or states of assembly as part of dynamic macromolecular complexes. This opens the door to real-time single-molecule analysis of the correlations between structure, function, and composition of large multicomponent protein complexes. (© 2017 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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