Functional consequences of co-expressing connexin40 or connexin45 with connexin43 on intercellular electrical coupling.

Autor: Thomas NM; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: neilmjthomas@gmail.com., Gray R; The Whittington Hospital NHS Trust, London, UK. Electronic address: rosaire.gray@nhs.net., Fry CH; School of Physiology, Pharmacology & Neuroscience, University of Bristol, Bristol, UK. Electronic address: chris.fry@bristol.ac.uk., Desplantez T; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: Thomas.Desplantez@ihu-liryc.fr., Peters NS; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: n.peters@imperial.ac.uk., Severs NJ; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: n.severs@imperial.ac.uk., Macleod KT; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: k.t.macleod@imperial.ac.uk., Dupont E; Myocardial Function, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address: e.dupont@imperial.ac.uk.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2017 Jan 29; Vol. 483 (1), pp. 191-196. Date of Electronic Publication: 2016 Dec 26.
DOI: 10.1016/j.bbrc.2016.12.169
Abstrakt: The functional characteristics of the co-expression of connexin43, connexin40, and connexin45 proteins in human myocardium are thought to play an important role in governing normal propagation of the cardiac electrical impulse and in generating the myocardial substrate for some arrhythmias and conduction disturbances. A rat liver epithelial cell line, that endogenously expresses connexin43, was used to induce also expression of connexin40 or connexin45 after stable transfection using an inducible ecdysone system. Electrical coupling was estimated from measurement of the input resistance of transfected cells using an intracellular microelectrode to inject current and record changes to membrane potential. However, varied expression of the transfected connexin40 or connexin45 did not change electrical coupling, although connexin43/40 co-expression led to better coupling than connexin43/45 co-expression. Quantification of endogenous connexin43 expression, at both mRNA and protein levels, showed that it was altered in a manner dependent on the transfected connexin isotype. The data using rat liver epithelial cells indicate an increased electrical coupling upon expression of connexin40 and connexin43 but decreased coupling with connexin45 and connexin43 co-expression.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: