Isoflavin-β modifies muscle oxidative stress and prevents a thyrotoxicosis-induced loss of muscle mass in rats.

Autor: Marinello PC; Laboratory of Molecular Pathology, State University of Londrina, Rodovia Celso Garcia Cid, PR445, Km 380 Campus Universitário, Londrina, CEP 86051-990, Paraná, Brazil., Bernardes SS; Laboratory of Molecular Pathology, State University of Londrina, Rodovia Celso Garcia Cid, PR445, Km 380 Campus Universitário, Londrina, CEP 86051-990, Paraná, Brazil., Guarnier FA; Laboratory of Pathophysiology and Muscle Adaptation, State University of Londrina, Londrina, Paraná, Brazil., Da Silva TNX; Laboratory of Pathophysiology and Muscle Adaptation, State University of Londrina, Londrina, Paraná, Brazil., Borges FH; Laboratory of Pathophysiology and Free Radicals, State University of Londrina, Londrina, Paraná, Brazil., Lopes NMD; Laboratory of Molecular Pathology, State University of Londrina, Rodovia Celso Garcia Cid, PR445, Km 380 Campus Universitário, Londrina, CEP 86051-990, Paraná, Brazil., Simão ANC; Department of Pathology, Clinical and Toxicological Analysis, University Hospital of State University of Londrina, Londrina, Paraná, Brazil., Armani A; Department of Surgery, University Hospital of State University of Londrina, Londrina, Paraná, Brazil., Cecchini R; Laboratory of Pathophysiology and Free Radicals, State University of Londrina, Londrina, Paraná, Brazil., Cecchini AL; Laboratory of Molecular Pathology, State University of Londrina, Rodovia Celso Garcia Cid, PR445, Km 380 Campus Universitário, Londrina, CEP 86051-990, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Muscle & nerve [Muscle Nerve] 2017 Nov; Vol. 56 (5), pp. 975-981. Date of Electronic Publication: 2017 Mar 24.
DOI: 10.1002/mus.25546
Abstrakt: Introduction: We sought to verify whether isoflavin-beta (Iso-β), a mixture of isoflavones with antioxidant properties, could prevent thyrotoxicosis-induced loss of muscle mass and the participation of oxidative stress (OS) in the mechanisms of this prevention.
Methods: Two experimental periods of thyrotoxicosis induction were used in Wistar rats: 3 and 5 days to assess Iso-β effects before and after thyrotoxicosis-induced muscle wasting. After euthanasia, peritoneal fat and gastrocnemius muscle were collected, weighed, and muscle OS was assessed.
Results: Iso-β prevented the loss of gastrocnemius mass in thyrotoxic rats through the prevention of muscle OS generation during thyrotoxicosis, increasing muscle total antioxidant capacity and decreasing mitochondrial cytochrome c oxidase activity, lipid peroxidation, and protein carbonyl content.
Conclusion: Iso-β decreased oxidative modification of proteins, which is known to exert a major role during proteolysis induction and is present in thyrotoxic myopathy, highlighting the potential action of Iso-β in this complication of the disease. Muscle Nerve 56: 975-981, 2017.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
Externí odkaz: