A lateral flow assay (LFA) for the rapid detection of extraparenchymal neurocysticercosis using cerebrospinal fluid.

Autor: Fleury A; Unidad de Neuroinflamación (Instituto de Investigaciones Biomédicas-UNAM, Instituto Nacional de Neurología y Neurocirugia, Facultad de Médicina-UNAM), Insurgentes Sur 3877, Colonia La Fama, delegación Tlalpan, Mexico D.F, Mexico; Neurocysticercosis Clinic, Instituto Nacional de Neurología y Neurocirugia, Mexico D.F, Mexico. Electronic address: afleury@biomedicas.unam.mx., Sastre P; Inmunología y Genética Aplicada S.A. (INGENASA), C/Hermanos García Noblejas 39, 28037 Madrid, Spain. Electronic address: psastre@ingenasa.com., Sciutto E; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico. Electronic address: edda@unam.mx., Correia S; Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal. Electronic address: scorreia@igc.gulbenkian.pt., Monedero A; Inmunología y Genética Aplicada S.A. (INGENASA), C/Hermanos García Noblejas 39, 28037 Madrid, Spain. Electronic address: amonedero@ingenasa.com., Toledo A; Unidad de Neuroinflamación (Instituto de Investigaciones Biomédicas-UNAM, Instituto Nacional de Neurología y Neurocirugia, Facultad de Médicina-UNAM), Insurgentes Sur 3877, Colonia La Fama, delegación Tlalpan, Mexico D.F, Mexico. Electronic address: antoro06@yahoo.com., Hernandez M; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico. Electronic address: marysel_01@yahoo.com.mx., Harrison LJ; University of Edinburgh, Royal (Dick) School of Veterinary Science, Easter Bush Veterinary Centre, Easter Bush, ROSLIN, Midlothian, EH259RG Scotland, UK. Electronic address: Leslie.Harrison@ed.ac.uk., Parkhouse RM; Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal. Electronic address: parkhous@igc.gulbenkian.pt.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2016 Oct 27. Date of Electronic Publication: 2016 Oct 27.
DOI: 10.1016/j.exppara.2016.10.016
Abstrakt: A lateral flow assay (LFA) for the diagnosis and monitoring of extraparenchymal neurocysticercosis, has been developed. The assay is based on the use of the monoclonal antibody HP10, and when applied to cerebrospinal fluid, correctly identified 34 cases of active extraparenchymal neurocysticercosis, but was negative with 26 samples from treated and cured neurocysticercosis patients and with 20 samples from unrelated neurological diseases. There was complete agreement between the HP10 Ag-ELISA results and the HP10-LFA. The HP10-LFA thus has utility for diagnosis and treatment of extraparenchymal neurocysticercosis, frequently a more dangerous form of the infection.
(Copyright © 2016. Published by Elsevier Inc.)
Databáze: MEDLINE
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