Nephroprotective effects of (-)-α-bisabolol against ischemic-reperfusion acute kidney injury.

Autor: Sampaio TL; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil., Menezes RR; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil. Electronic address: ramonppessoa@gmail.com., da Costa MF; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil., Meneses GC; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil., Arrieta MC; Instituto Clodomiro Picado, Universidad de Costa Rica, San José, Costa Rica., Chaves Filho AJ; Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará, Brazil., de Morais GB; School of Veterinar y Medicine, State University of Ceará, Fortaleza, Ceará, Brazil., Libório AB; Department of Clinical Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Ceara, Brazil., Alves RS; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil., Evangelista JS; School of Veterinar y Medicine, State University of Ceará, Fortaleza, Ceará, Brazil., Martins AM; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Jazyk: angličtina
Zdroj: Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2016 Dec 15; Vol. 23 (14), pp. 1843-1852. Date of Electronic Publication: 2016 Nov 11.
DOI: 10.1016/j.phymed.2016.11.008
Abstrakt: Background: Ischemia/reperfusion (I/R) in kidney is commonly related to acute kidney injury (AKI), essentially through oxidative stress. (-)-α-Bisabolol is a sesquiterpene isolated from the essential oil of a variety of plants, including chamomile, which has important antioxidant activity.
Study Design: This study intends to evaluate the nephroprotective activity of (-)-α-bisabolol (Bis) in both in vivo and in vitro models of kidney I/R.
Methods: Male Wistar rats were submitted to right nephrectomy, followed by ischemia by clamping of the renal artery in the left kidney for 60min. and 48h of reperfusion. The animals were treated orally with Bis (100mg/kg) or vehicle for 24h after reperfusion, and placed in metabolic cages, to evaluate water consumption, diuresis, urinary osmolality, classic biochemical markers and urinary KIM-1 (kidney injury molecule-1). Additionally, the left kidney was collected for histological evaluation and determination of glutathione (GSH) and Thiobarbituric Acid Reactive Substances (TBARS) levels. Tubular epithelial cells LLC-MK2 were used to assess Bis effect on in vitro I/R, by MTT assay. It was performed the cellular respiration tests by flow cytometry: evaluation of the production of cytoplasmic reactive oxygen species by DCFH-DA assay and mitochondrial transmembrane potential analysis with the dye rhodamine 123.
Results: I/R caused alterations in diuresis, water intake, urinary osmolality, plasmatic creatinine, urea and uric acid, creatinine clearance, proteinuria and microalbuminuria. Treatment with Bis ameliorated all of these parameters. Also, KIM-1 level enhanced by I/R was also diminished in groups treated with Bis. The histological examination showed that Bis attenuated the morphological changes caused by I/R, markedly vascular congestion and intratubular deposits of proteinaceous material. Additionally, Bis was able to reduce the changes observed in TBARS and GSH levels in kidney tissue. In in vitro assay, Bis was capable to partially protect the cell lineage against cell damage induced by I/R.
Conclusion: (-)-α-Bisabolol has a nephroprotective effect in kidney I/R, with antioxidant effect. Moreover, this result seems to be associated to a direct protective effect on tubular epithelia.
(Copyright © 2016 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: