Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques.

Autor:	Aliota MT; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Dudley DM; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Newman CM; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Mohr EL; Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, United States of America., Gellerup DD; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Breitbach ME; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Buechler CR; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Rasheed MN; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Mohns MS; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Weiler AM; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Barry GL; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Weisgrau KL; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Eudailey JA; Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America., Rakasz EG; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Vosler LJ; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Post J; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Capuano S 3rd; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Golos TG; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Departments of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Departments of Obstetrics and Gynecology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Permar SR; Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America.; Department of Pediatrics, Duke University Medical Center, Durham, North Carolina, United States of America., Osorio JE; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Friedrich TC; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., O'Connor SL; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., O'Connor DH; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
Jazyk:	angličtina
Zdroj:	PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2016 Dec 02; Vol. 10 (12), pp. e0005168. Date of Electronic Publication: 2016 Dec 02 (Print Publication: 2016).
DOI:	10.1371/journal.pntd.0005168
Abstrakt:	Background: Zika virus (ZIKV; Flaviviridae, Flavivirus) was declared a public health emergency of international concern by the World Health Organization (WHO) in February 2016, because of the evidence linking infection with ZIKV to neurological complications, such as Guillain-Barre Syndrome in adults and congenital birth defects including microcephaly in the developing fetus. Because development of a ZIKV vaccine is a top research priority and because the genetic and antigenic variability of many RNA viruses limits the effectiveness of vaccines, assessing whether immunity elicited against one ZIKV strain is sufficient to confer broad protection against all ZIKV strains is critical. Recently, in vitro studies demonstrated that ZIKV likely circulates as a single serotype. Here, we demonstrate that immunity elicited by African lineage ZIKV protects rhesus macaques against subsequent infection with Asian lineage ZIKV. Methodology/principal Findings: Using our recently developed rhesus macaque model of ZIKV infection, we report that the prototypical ZIKV strain MR766 productively infects macaques, and that immunity elicited by MR766 protects macaques against heterologous Asian ZIKV. Furthermore, using next generation deep sequencing, we found in vivo restoration of a putative N-linked glycosylation site upon replication in macaques that is absent in numerous MR766 strains that are widely being used by the research community. This reversion highlights the importance of carefully examining the sequence composition of all viral stocks as well as understanding how passage history may alter a virus from its original form. Conclusions/significance: An effective ZIKV vaccine is needed to prevent infection-associated fetal abnormalities. Macaques whose immune responses were primed by infection with East African ZIKV were completely protected from detectable viremia when subsequently rechallenged with heterologous Asian ZIKV. Therefore, these data suggest that immunogen selection is unlikely to adversely affect the breadth of vaccine protection, i.e., any Asian ZIKV immunogen that protects against homologous challenge will likely confer protection against all other Asian ZIKV strains. Competing Interests: The authors have declared that no competing interests exist.
Databáze:	MEDLINE
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