Loss of Inositol Phosphorylceramide Sphingolipid Mannosylation Induces Plant Immune Responses and Reduces Cellulose Content in Arabidopsis.

Autor:	Fang L; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Ishikawa T; Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan., Rennie EA; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Murawska GM; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Lao J; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Yan J; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Tsai AY; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Baidoo EE; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720., Xu J; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720., Keasling JD; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720.; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720., Demura T; Cellulose Production Research Team, Biomass Engineering Program, Center for Sustainable Resource Science, RIKEN, Yokohama 230-0045, Japan.; Graduate School of Biological Sciences, Nara Institute of Science and Technology, 630-0192 Nara, Japan., Kawai-Yamada M; Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan., Scheller HV; Joint BioEnergy Institute, Emeryville, California 94608.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720.; Plant and Microbial Biology, University of California, Berkeley, California 94720., Mortimer JC; Joint BioEnergy Institute, Emeryville, California 94608 jcmortimer@lbl.gov.; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720.; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2016 Dec; Vol. 28 (12), pp. 2991-3004. Date of Electronic Publication: 2016 Nov 28.
DOI:	10.1105/tpc.16.00186
Abstrakt:	Glycosylinositol phosphorylceramides (GIPCs) are a class of glycosylated sphingolipids found in plants, fungi, and protozoa. These lipids are abundant in the plant plasma membrane, forming ∼25% of total plasma membrane lipids. Little is known about the function of the glycosylated headgroup, but two recent studies have indicated that they play a key role in plant signaling and defense. Here, we show that a member of glycosyltransferase family 64, previously named ECTOPICALLY PARTING CELLS1, is likely a Golgi-localized GIPC-specific mannosyl-transferase, which we renamed GIPC MANNOSYL-TRANSFERASE1 (GMT1). Sphingolipid analysis revealed that the Arabidopsis thaliana gmt1 mutant almost completely lacks mannose-carrying GIPCs. Heterologous expression of GMT1 in Saccharomyces cerevisiae and tobacco (Nicotiana tabacum) cv Bright Yellow 2 resulted in the production of non-native mannosylated GIPCs. gmt1 displays a severe dwarfed phenotype and a constitutive hypersensitive response characterized by elevated salicylic acid and hydrogen peroxide levels, similar to that we previously reported for the Golgi-localized, GIPC-specific, GDP-Man transporter GONST1 (Mortimer et al., 2013). Unexpectedly, we show that gmt1 cell walls have a reduction in cellulose content, although other matrix polysaccharides are unchanged. (© 2016 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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