| Autor: |
Bormotova EA; Department of Molecular Microbiology Institute of Experimental Medicine, St. Petersburg, Russia., Gupalova TV; Department of Molecular Microbiology Institute of Experimental Medicine, St. Petersburg, Russia. tvgupalova@rambler.ru. |
| Jazyk: |
angličtina |
| Zdroj: |
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Nov; Vol. 162 (1), pp. 66-70. Date of Electronic Publication: 2016 Nov 23. |
| DOI: |
10.1007/s10517-016-3547-3 |
| Abstrakt: |
Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protein G from group G and C Streptococcus strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G Streptococcus of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink