Extracellular Vesicles Isolated from the Leaf Apoplast Carry Stress-Response Proteins.
| Autor: | Rutter BD; Department of Biology, Indiana University, Bloomington, Indiana 47405., Innes RW; Department of Biology, Indiana University, Bloomington, Indiana 47405 rinnes@indiana.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Plant physiology [Plant Physiol] 2017 Jan; Vol. 173 (1), pp. 728-741. Date of Electronic Publication: 2016 Nov 08. |
| DOI: | 10.1104/pp.16.01253 |
| Abstrakt: | Exosomes are extracellular vesicles (EVs) that play a central role in intercellular signaling in mammals by transporting proteins and small RNAs. Plants are also known to produce EVs, particularly in response to pathogen infection. The contents of plant EVs have not been analyzed, however, and their function is unknown. Here, we describe a method for purifying EVs from the apoplastic fluids of Arabidopsis (Arabidopsis thaliana) leaves. Proteomic analyses of these EVs revealed that they are highly enriched in proteins involved in biotic and abiotic stress responses. Consistent with this finding, EV secretion was enhanced in plants infected with Pseudomonas syringae and in response to treatment with salicylic acid. These findings suggest that EVs may represent an important component of plant immune responses. (© 2017 American Society of Plant Biologists. All Rights Reserved.) |
| Databáze: | MEDLINE |
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