Congenital Toxoplasmosis in Chronically Infected and Subsequently Challenged Ewes.

Autor: Dos Santos TR; Department of Veterinary Medicine, Rondônia Federal University (UNIR), Rolim de Moura, Av. Norte-Sul, 7300 - Rolim de Moura, RO, 76940-000, Brazil., Faria GD; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil., Guerreiro BM; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil., Dal Pietro NH; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil., Lopes WD; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil., da Silva HM; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil., Garcia JL; Department of Preventive Veterinary Medicine, UEL - Londrina, SP, Brazil., Luvizotto MC; Department of Clinic, Surgery and Animal Reproduction, FMVA, UNESP - Araçatuba, SP, Brazil., Bresciani KD; Department of Production and Animal Health, FMVA, UNESP - Araçatuba, SP, Brazil., da Costa AJ; Department of Animal Pathology, CPPAR, FCAV, UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/n - Jaboticabal, SP, 14884-900, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Oct 27; Vol. 11 (10), pp. e0165124. Date of Electronic Publication: 2016 Oct 27 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0165124
Abstrakt: This experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz: