Plasma miR-19b and miR-183 as Potential Biomarkers of Lung Cancer.

Autor: Zaporozhchenko IA; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia.; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia., Morozkin ES; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia.; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia., Skvortsova TE; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia., Ponomaryova AA; Tomsk Cancer Research Institute of SB RAMS, Tomsk, Russia.; National Research Tomsk Polytechnic University, Tomsk, Russia., Rykova EY; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia., Cherdyntseva NV; Tomsk Cancer Research Institute of SB RAMS, Tomsk, Russia.; National Research Tomsk State University, Tomsk, Russia., Polovnikov ES; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia., Pashkovskaya OA; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia., Pokushalov EA; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia., Vlassov VV; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia., Laktionov PP; Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia.; Novosibirsk Research Institute of Circulation Pathology of Academician E.N. Meshalkin, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Oct 21; Vol. 11 (10), pp. e0165261. Date of Electronic Publication: 2016 Oct 21 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0165261
Abstrakt: Lung cancer is a complex disease that often manifests at the point when treatment is not effective. Introduction of blood-based complementary diagnostics using molecular markers may enhance early detection of this disease and help reduce the burden of lung cancer. Here we evaluated the diagnostic potential of seven plasma miRNA biomarkers (miR-21, -19b, -126, -25, -205, -183, -125b) by quantitative reverse transcription PCR. Influence clinical and demographical characteristics, including age, tumor stage and cancer subtype on miRNA levels was investigated. Four miRNAs were significantly dysregulated (miR-19b, -21, -25, -183) in lung cancer patients. Combination of miR-19b and miR-183 provided detection of lung cancer with 94.7% sensitivity and 95.2% specificity (AUC = 0.990). Thus, miRNAs have shown the potential to discriminate histological subtypes of lung cancer and reliably distinguish lung cancer patients from healthy individuals.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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