Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing.

Autor:	Jerônimo MS; PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, Universidade de Brasília (UnB), Brazil. Design of the study, technical precedures, statistical analysis, manuscript writing., Barros AD; Fellow PhD degree, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures., MoritaI VE; MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures., Alves EO; MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures., Souza NL; MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures., Almeida RM; PhD, Associate Professor, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures, analysis of data., Nóbrega YK; Full Professor, Pathology Department, Faculty of Medicine, UnB, Brasilia-DF, Brazil. Technical procedures, statistical analysis, analysis of data., Cavalcanti FF Neto; PhD, Associate Professor, Pathology Department, Faculty of Medicine, UnB, Brasilia-DF, Brazil. Technical procedures, statistical analysis, analysis of data., Amorin R; PhD, Associate Professor, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures, analysis of data., Borin MF; PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, UnB, Brasilia-DF, Brazil. Design of the study, statistical analysis, analysis of data, manuscript writing., Bocca AL; PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, UnB, Brasilia-DF, Brazil. Design of the study, statistical analysis, analysis of data, manuscript writing.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2016 Sep; Vol. 31 (9), pp. 586-596.
DOI:	10.1590/S0102-865020160090000003
Abstrakt:	Purpose:: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. Methods:: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. Results:: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. Conclusion:: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu