| Autor: |
Biswas S; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Fan W; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Li R; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Li S; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Ping W; Institute of Tobacco Research, Henan Academy of Agricultural Sciences, Xuchang, Henan 461000, China., Li S; Institute of Tobacco Research, Henan Academy of Agricultural Sciences, Xuchang, Henan 461000, China., Naumova A; Central Customs Laboratory, Bulgarian Customs Agency, G. S. Rakovski Street 47, 1202 Sofia, Bulgaria., Peelen T; Dutch Customs Laboratory, Kingsfordweg 1, 1043 GN Amsterdam, Netherlands., Kok E; RIKILT, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, Netherlands., Yuan Z; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Zhang D; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China., Shi J; Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China. |
| Abstrakt: |
Reliable methods are needed to detect the presence of tobacco components in tobacco products to effectively control smuggling and classify tariff and excise in tobacco industry to control illegal tobacco trade. In this study, two sensitive and specific DNA based methods, one quantitative real-time PCR (qPCR) assay and the other loop-mediated isothermal amplification (LAMP) assay, were developed for the reliable and efficient detection of the presence of tobacco (Nicotiana tabacum) in various tobacco samples and commodities. Both assays targeted the same sequence of the uridine 5'-monophosphate synthase (UMPS), and their specificities and sensitivities were determined with various plant materials. Both qPCR and LAMP methods were reliable and accurate in the rapid detection of tobacco components in various practical samples, including customs samples, reconstituted tobacco samples, and locally purchased cigarettes, showing high potential for their application in tobacco identification, particularly in the special cases where the morphology or chemical compositions of tobacco have been disrupted. Therefore, combining both methods would facilitate not only the detection of tobacco smuggling control, but also the detection of tariff classification and of excise. |
