Evaluation of Virulence Gene Expression Patterns in Acinetobacter baumannii Using Quantitative Real-Time Polymerase Chain Reaction Array.

Autor: Lannan FM; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., O'conor DK; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Broderick JC; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Tate JF; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Scoggin JT; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Moran NA; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Husson CM; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Hegeman EM; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Ogrydziak CE; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Singh SA; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Vafides AG; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Brinkley CC; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905., Goodin JL; Department of Chemistry and Life Science, United States Military Academy, 646 Swift Road, West Point, New York 10996-1905.
Jazyk: angličtina
Zdroj: Military medicine [Mil Med] 2016 Sep; Vol. 181 (9), pp. 1108-13.
DOI: 10.7205/MILMED-D-15-00437
Abstrakt: According to the Centers for Disease Control's recently devised National Strategy for Combating Antibiotic-Resistant Bacteria, Acinetobacter baumannii is a "serious" threat level pathogen. A. baumannii's notoriety stems from the fact that a large number of modern strains are multidrug resistant and persist in the hospital setting, thus causing numerous deaths per year. It is imperative that research focus on a more fundamental understanding of the factors responsible for the success of A. baumannii. Toward this end, our group investigated virulence gene expression patterns in a recently characterized wound isolate, AB5075, using quantitative real-time polymerase chain reaction array. Notably, several genes showed statistically significant upregulation at 37°C compared to 25°C; MviM, Wbbj, CarO, and certain genes of the Bas, Bar, and Csu operons. Additionally, we found that in vitro biofilm formation by Csu transposon insertion mutant strains is attenuated. These findings validate previous reports that suggest a link between the Csu operon and biofilm formation. More importantly, our results demonstrate a successful method for evaluating the significance of previously identified virulence factors in a modern and clinically relevant strain of A. baumannii, thereby providing a path toward a more fundamental understanding of the pathogenicity of A. baumannii.
(Reprint & Copyright © 2016 Association of Military Surgeons of the U.S.)
Databáze: MEDLINE
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