Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain.

Autor: Vale VL; Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil. vvale@uneb.br.; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil. vvale@uneb.br., Silva Mda C; Department of Life Sciences, State University of Bahia, Rua Silveira Martins 2555, Cabula, Salvador, BA, CEP 41150-000, Brazil.; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., de Souza AP; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., Trindade SC; Department of Health, Feira de Santana State University, Avenida Transnordestina s/n, Novo Horizonte, Feira de Santana, BA, CEP 44036-900, Brazil.; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., de Moura-Costa LF; Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., Dos Santos-Lima EK; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., Nascimento IL; Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., Cardoso HS; Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil., Marques Ede J; Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil., Paule BJ; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil., Nascimento RJ; Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.; Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2016 Sep 08; Vol. 12 (1), pp. 195. Date of Electronic Publication: 2016 Sep 08.
DOI: 10.1186/s12917-016-0811-8
Abstrakt: Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.
Results: The lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.
Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x10(7) CFU, no signs of the disease were observed.
Databáze: MEDLINE
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