Tumor Eradication by Cisplatin Is Sustained by CD80/86-Mediated Costimulation of CD8+ T Cells.

Autor:	Beyranvand Nejad E; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., van der Sluis TC; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., van Duikeren S; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Yagita H; Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan., Janssen GM; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands., van Veelen PA; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands., Melief CJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands. ISA Pharmaceuticals, Leiden, the Netherlands., van der Burg SH; Department of Clinical Oncology, Leiden University Medical Center, Leiden, the Netherlands., Arens R; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands. R.Arens@lumc.nl.
Jazyk:	angličtina
Zdroj:	Cancer research [Cancer Res] 2016 Oct 15; Vol. 76 (20), pp. 6017-6029. Date of Electronic Publication: 2016 Aug 28.
DOI:	10.1158/0008-5472.CAN-16-0881
Abstrakt:	Certain cytotoxic chemotherapeutic drugs are immunogenic, stimulating tumor immunity through mechanisms that are not completely understood. Here we show how the DNA-damaging drug cisplatin modulates tumor immunity. At the maximum tolerated dose (MTD), cisplatin cured 50% of mice with established murine TC-1 or C3 tumors, which are preclinical models of human papillomavirus (HPV)-associated cancer. Notably, the curative benefit of cisplatin relied entirely upon induction of tumor-specific CD8 + T cells. Mechanistic investigations showed that cisplatin stimulated tumor infiltration of inflammatory antigen-presenting cells (APC) expressing relatively higher levels of the T-cell costimulatory ligands CD70, CD80, and CD86. Cell death triggered by cisplatin was associated with the release of at least 19 proteins in the tumor environment that could act as damage-associated molecular patterns and upregulate costimulatory molecules, either alone or in concert, but the responsible proteins remain unknown. Essentially, the curative effect of cisplatin was abrogated in mice lacking expression of CD80 and CD86 on APCs. Furthermore, cisplatin treatment was improved by CTLA-4 blockade, which increases the availability of CD80/86 to bind to CD28. In contrast, there was no effect of CD27 stimulation, which replaces CD70 interaction. At the cisplatin MTD, cure rates could also be increased by vaccination with synthetic long peptides, whereas cures could also be achieved at similar rates at 80% of the MTD with reduced side effects. Our findings reveal an essential basis for the immunogenic properties of cisplatin, which are mediated by the induction of costimulatory signals for CD8 + T-cell-dependent tumor destruction. Cancer Res; 76(20); 6017-29. ©2016 AACR. (©2016 American Association for Cancer Research.)
Databáze:	MEDLINE
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