Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis.

Autor: Shin SH; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia., Hsu A; Emory College, Emory University, Atlanta, Georgia., Chastain HM; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia., Cruz LA; Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico., Elder ES; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia., Sapp SG; University of Georgia, Athens, Georgia., McAuliffe I; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia., Espino AM; Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico., Handali S; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia. ahi0@cdc.gov.
Jazyk: angličtina
Zdroj: The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2016 Oct 05; Vol. 95 (4), pp. 852-855. Date of Electronic Publication: 2016 Aug 22.
DOI: 10.4269/ajtmh.16-0253
Abstrakt: In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)-FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG 4 antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG 4 WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG 4 , the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis.
(© The American Society of Tropical Medicine and Hygiene.)
Databáze: MEDLINE
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