The time-resolved transcriptome of C. elegans.

Autor:	Boeck ME; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA; Department of Biology, Regis University, Denver, Colorado 80221, USA., Huynh C; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA., Gevirtzman L; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA., Thompson OA; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA., Wang G; Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06520, USA., Kasper DM; Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06520, USA., Reinke V; Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06520, USA., Hillier LW; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA., Waterston RH; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA.
Jazyk:	angličtina
Zdroj:	Genome research [Genome Res] 2016 Oct; Vol. 26 (10), pp. 1441-1450. Date of Electronic Publication: 2016 Aug 16.
DOI:	10.1101/gr.202663.115
Abstrakt:	We generated detailed RNA-seq data for the nematode Caenorhabditis elegans with high temporal resolution in the embryo as well as representative samples from post-embryonic stages across the life cycle. The data reveal that early and late embryogenesis is accompanied by large numbers of genes changing expression, whereas fewer genes are changing in mid-embryogenesis. This lull in genes changing expression correlates with a period during which histone mRNAs produce almost 40% of the RNA-seq reads. We find evidence for many more splice junctions than are annotated in WormBase, with many of these suggesting alternative splice forms, often with differential usage over the life cycle. We annotated internal promoter usage in operons using SL1 and SL2 data. We also uncovered correlated transcriptional programs that span >80 kb. These data provide detailed annotation of the C. elegans transcriptome. (© 2016 Boeck et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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