Multi-class analysis of new psychoactive substances and metabolites in hair by pressurized liquid extraction coupled to HPLC-HRMS.

Autor: Montesano C; Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy., Vannutelli G; Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy., Massa M; Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy., Simeoni MC; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023, Mosciano Sant'Angelo, TE, Italy., Gregori A; Department of Scientific Investigation (RIS), Carabinieri, 00191, Rome, Italy., Ripani L; Department of Scientific Investigation (RIS), Carabinieri, 00191, Rome, Italy., Compagnone D; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023, Mosciano Sant'Angelo, TE, Italy., Curini R; Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy., Sergi M; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023, Mosciano Sant'Angelo, TE, Italy.
Jazyk: angličtina
Zdroj: Drug testing and analysis [Drug Test Anal] 2017 May; Vol. 9 (5), pp. 798-807. Date of Electronic Publication: 2016 Aug 08.
DOI: 10.1002/dta.2043
Abstrakt: In this paper, an analytical method has been developed and validated for the analysis of new psychoactive substances (NPS) and metabolites in hair samples. The method was based on pressurized liquid extraction (PLE) followed by solid-phase extraction (SPE) clean-up and high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) analysis. To evaluate extraction efficiency and the applicability of the method, hair samples were fortified by soaking in order to obtain a good surrogate for drug users' hair; the amount of incorporated drugs related to their lipophilicity, similarly to in vivo drug incorporation. To the best of our knowledge, this is the first method that allowed for the analysis of both cathinones (5) and synthetic cannabinoids (7) in hair with a single extraction procedure and chromatographic run. A phenethylamine (2C-T-4), 4- fluorophenylpiperazine and methoxetamine were also included showing that PLE coupled to SPE clean-up was suitable for a multi-class analysis of NPS in hair. In addition, the use of PLE significantly reduced hair analysis time: decontamination, incubation, clean-up, and liquid chromatography-mass spectrometry (LC-MS) analysis were carried out in approximately 45 min. The method was fully validated according to Scientific Working Group for Forensic Toxicology (SWGTOX) and Society of Hair Testing (SoHT) guidelines. Limit of quantification (LOQ) values ranged from 8 to 50 pg mg -1 for cathinones, phenetylamines and piperazines, and from 9 to 40 pg mg -1 for synthetic cannabinoids (10 pg mg -1 for methoxetamine). Matrix effects were below 15% for all the analytes, demonstrating the effectiveness of the clean-up step. Inaccuracy was lower than 9% in terms of bias. Copyright © 2016 John Wiley & Sons, Ltd.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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