[Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells].

Autor:	Kozlova NI; Institute of Biomedical Chemistry, Moscow, Russia., Morozevich GE; Institute of Biomedical Chemistry, Moscow, Russia., Ushakova NA; Institute of Biomedical Chemistry, Moscow, Russia., Gevorkian NM; Institute of Biomedical Chemistry, Moscow, Russia., Berman AE; Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2016 Mar; Vol. 62 (3), pp. 272-8.
DOI:	10.18097/PBMC20166203272
Abstrakt:	In MCF-7/Dox human breast carcinoma cells, down-regulation of integrin alpha5beta1 and inhibition of epidermal growth factor receptor (EGFR) markedly reduced rates of cell proliferation. Mitotic cycle analysis showed that alpha5beta1 down-regulation resulted in cell cycle arrest at the S phase, followed by a significant increase in the population of apoptotic cells (subG1 population). Inhibition of EGFR activity also caused cell cycle arrest at the S-phase but without any increase in the subG1 population. Down-regulation of alpha5beta1 and EGFR inhibition resulted in a significant decrease of cell content of the active (phosphorylated) forms of FAK and Erk protein kinases. The data obtained suggest that alpha5beta1 integrin is implicated in cell growth control via inhibition of apoptotic cell death and through EGFR activation.
Databáze:	MEDLINE
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