Quantitation of IgG antibody to Streptococcus pneumoniae vaccine by ELISA and FAST-ELISA using tyraminated antigen.

Autor: Boctor FN; Specialty Laboratories, Inc., Santa Monica, CA 90404., Barka NE, Agopian MS
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 1989 Jun 21; Vol. 120 (2), pp. 167-71.
DOI: 10.1016/0022-1759(89)90238-x
Abstrakt: We examined two ELISA methods for measuring antibodies to Streptococcus pneumoniae using tyraminated S. pneumoniae polysaccharide types 3, 7N, 9F and 14 as antigens. The ELISA has the usual format with a relatively long incubation time whereas the FAST-ELISA has a short incubation time and employs a different solid-phase configuration. We showed that both techniques can be used for the detection of antibodies to S. pneumoniae polysaccharides. Although its analytical sensitivity is about 1/10 of that of the ELISA, the FAST-ELISA is sufficiently sensitive to distinguish protective from unprotective levels of antibodies to the types of S. pneumoniae studied. In studying pre- and post-immunization response, we showed that type 3 is the most immunogenic.
Databáze: MEDLINE