| Autor: |
Boctor FN; Specialty Laboratories, Inc., Santa Monica, CA 90404., Barka NE, Agopian MS |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1989 Jun 21; Vol. 120 (2), pp. 167-71. |
| DOI: |
10.1016/0022-1759(89)90238-x |
| Abstrakt: |
We examined two ELISA methods for measuring antibodies to Streptococcus pneumoniae using tyraminated S. pneumoniae polysaccharide types 3, 7N, 9F and 14 as antigens. The ELISA has the usual format with a relatively long incubation time whereas the FAST-ELISA has a short incubation time and employs a different solid-phase configuration. We showed that both techniques can be used for the detection of antibodies to S. pneumoniae polysaccharides. Although its analytical sensitivity is about 1/10 of that of the ELISA, the FAST-ELISA is sufficiently sensitive to distinguish protective from unprotective levels of antibodies to the types of S. pneumoniae studied. In studying pre- and post-immunization response, we showed that type 3 is the most immunogenic. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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