The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF.

Autor: Zhou S; Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: zhou@sund.ku.dk., Ochalek A; BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent Istvan University, Gödöllő, Hungary. Electronic address: Anna.Ochalek@biotalentum.hu., Szczesna K; BioTalentum Ltd., Gödöllő, Hungary. Electronic address: Karolina.Szczesna@biotalentum.hu., Avci HX; BioTalentum Ltd., Gödöllő, Hungary; Department of Medical Chemistry, University of Szeged, Dóm tér 8, 6720 Szeged, Hungary. Electronic address: Hasan.Avci@biotalentum.hu., Kobolák J; BioTalentum Ltd., Gödöllő, Hungary. Electronic address: Julianna.Kobolak@biotalentum.hu., Varga E; BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent Istvan University, Gödöllő, Hungary. Electronic address: soszter@gmail.com., Rasmussen M; Bioneer A/S, Hoersholm, Denmark. Electronic address: mar@bioneer.dk., Holst B; Bioneer A/S, Hoersholm, Denmark. Electronic address: bho@bioneer.dk., Cirera S; Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: scs@sund.ku.dk., Hyttel P; Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: poh@sund.ku.dk., Freude KK; Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: kkf@sund.ku.dk., Dinnyés A; BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent Istvan University, Gödöllő, Hungary; Departments of Equine Sciences and Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands. Electronic address: andras.dinnyes@biotalentum.hu.
Jazyk: angličtina
Zdroj: Differentiation; research in biological diversity [Differentiation] 2016 Oct - Nov; Vol. 92 (4), pp. 183-194. Date of Electronic Publication: 2016 Jun 16.
DOI: 10.1016/j.diff.2016.06.002
Abstrakt: Neural rosettes derived from human induced pluripotent stem cells (iPSCs) have been claimed to be a highly robust in vitro cellular model for biomedical application. They are able to propagate in vitro in the presence of mitogens, including basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). However, these two mitogens are also involved in anterior-posterior patterning in a gradient dependent manner along the neural tube axis. Here, we compared the regional identity of neural rosette cells and specific neural subtypes of their progeny propagated with low and high concentrations of bFGF and EGF. We observed that low concentrations of bFGF and EGF in the culturing system were able to induce forebrain identity of the neural rosettes and promote subsequent cortical neuronal differentiation. On the contrary, high concentrations of these mitogens stimulate a mid-hindbrain fate of the neural rosettes, resulting in subsequent cholinergic neuron differentiation. Thus, our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells.
(Copyright © 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: