Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuant(®) system.

Autor: Ewing MM; Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA. Electronic address: margaret.ewing@promega.com., Thompson JM; Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA. Electronic address: jonelle.thompson@promega.com., McLaren RS; Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA. Electronic address: bob.mclaren@promega.com., Purpero VM; Wisconsin State Crime Laboratory ∧ Madison, 4626 University Ave., Madison, WI 53705, USA. Electronic address: purperovm@doj.state.wi.us., Thomas KJ; Wisconsin State Crime Laboratory ∧ Madison, 4626 University Ave., Madison, WI 53705, USA. Electronic address: thomaskj@doj.state.wi.us., Dobrowski PA; Wisconsin State Crime Laboratory ∧ Milwaukee, 1578S 11th St., Milwaukee, WI 53204-2860, USA. Electronic address: dobrowskipa@doj.state.wi.us., DeGroot GA; Wisconsin State Crime Laboratory ∧ Milwaukee, 1578S 11th St., Milwaukee, WI 53204-2860, USA. Electronic address: degrootga@doj.state.wi.us., Romsos EL; U.S. National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899-8314, USA. Electronic address: erica.romsos@nist.gov., Storts DR; Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA. Electronic address: doug.storts@promega.com.
Jazyk: angličtina
Zdroj: Forensic science international. Genetics [Forensic Sci Int Genet] 2016 Jul; Vol. 23, pp. 166-177. Date of Electronic Publication: 2016 Apr 16.
DOI: 10.1016/j.fsigen.2016.04.007
Abstrakt: Quantification of the total amount of human DNA isolated from a forensic evidence item is crucial for DNA normalization prior to short tandem repeat (STR) DNA analysis and a federal quality assurance standard requirement. Previous commercial quantification methods determine the total human DNA and total human male DNA concentrations, but provide limited information about the condition of the DNA sample. The PowerQuant(®) System includes targets for quantification of total human and total human male DNA as well as targets for evaluating whether the human DNA is degraded and/or PCR inhibitors are present in the sample. A developmental validation of the PowerQuant(®) System was completed, following SWGDAM Validation Guidelines, to evaluate the assay's specificity, sensitivity, precision and accuracy, as well as the ability to detect degraded DNA or PCR inhibitors. In addition to the total human DNA and total human male DNA concentrations in a sample, data from the degradation target and internal PCR control (IPC) provide a forensic DNA analyst meaningful information about the quality of the isolated human DNA and the presence of PCR inhibitors in the sample that can be used to determine the most effective workflow and assist downstream interpretation.
(Copyright © 2016 The Author(s). Published by Elsevier Ireland Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: