| Autor: |
Kinahan DJ; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland., Kearney SM; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland., Kilcawley NA; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland., Early PL; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland., Glynn MT; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland., Ducrée J; School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.; Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland. |
| Abstrakt: |
Here we present retrieval of Peripheral Blood Mononuclear Cells by density-gradient medium based centrifugation for subsequent analysis of the leukocytes on an integrated microfluidic "Lab-on-a-Disc" cartridge. Isolation of white blood cells constitutes a critical sample preparation step for many bioassays. Centrifugo-pneumatic siphon valves are particularly suited for blood processing as they function without need of surface treatment and are 'low-pass', i.e., holding at high centrifugation speeds and opening upon reduction of the spin rate. Both 'hydrostatically' and 'hydrodynamically' triggered centrifugo-pneumatic siphon valving schemes are presented. Firstly, the geometry of the pneumatic chamber of hydrostatically primed centrifugo-pneumatic siphon valves is optimised to enable smooth and uniform layering of blood on top of the density-gradient medium; this feature proves to be key for efficient Peripheral Blood Mononuclear Cell extraction. A theoretical analysis of hydrostatically primed valves is also presented which determines the optimum priming pressure for the individual valves. Next, 'dual siphon' configurations for both hydrostatically and hydrodynamically primed centrifugo-pneumatic siphon valves are introduced; here plasma and Peripheral Blood Mononuclear Cells are extracted through a distinct siphon valve. This work represents a first step towards enabling on disc multi-parameter analysis. Finally, the efficiency of Peripheral Blood Mononuclear Cells extraction in these structures is characterised using a simplified design. A microfluidic mechanism, which we termed phase switching, is identified which affects the efficiency of Peripheral Blood Mononuclear Cell extraction. |
