Iron deficiency resistance mechanisms enlightened by gene expression analysis in Paenibacillus riograndensis SBR5.

Autor: Sperb ER; Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Prédio 43312, CEP 91501-970, Porto Alegre, RS, Brazil. Electronic address: edilena.sperb.1987@gmail.com., Tadra-Sfeir MZ; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Centro Politécnico, C. P. 19046, CEP 81531-980, Curitiba, PR, Brazil. Electronic address: miztadra@yahoo.com.br., Sperotto RA; Setor de Genética e Biologia Molecular do Museu de Ciências Naturais (MCN), Centro de Ciências Biológicas e da Saúde (CCBS), Programa de Pós-Graduação em Biotecnologia (PPGBiotec), Centro Universitário UNIVATES, Lajeado, RS, Brazil. Electronic address: raulsperotto@yahoo.com.br., Fernandes Gde C; Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Prédio 43312, CEP 91501-970, Porto Alegre, RS, Brazil. Electronic address: gabrieladcf@yahoo.com.br., Pedrosa Fde O; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Centro Politécnico, C. P. 19046, CEP 81531-980, Curitiba, PR, Brazil. Electronic address: fpedrosa@ufpr.br., de Souza EM; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Centro Politécnico, C. P. 19046, CEP 81531-980, Curitiba, PR, Brazil. Electronic address: souzaem@ufpr.br., Passaglia LM; Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Prédio 43312, CEP 91501-970, Porto Alegre, RS, Brazil. Electronic address: luciane.passaglia@ufrgs.br.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2016 Jul-Aug; Vol. 167 (6), pp. 501-9. Date of Electronic Publication: 2016 Apr 26.
DOI: 10.1016/j.resmic.2016.04.007
Abstrakt: Despite its importance in growth and cell division, iron metabolism is still poorly understood in microorganisms, especially in Gram-positive bacteria. In this work, we used RNA sequencing technology to elucidate global mechanisms involved in iron starvation resistance in Paenibacillus riograndensis SBR5, a potential plant growth-promoting bacterium. Iron deficiency caused several changes in gene expression, and 150 differentially expressed genes were found: 71 genes were overexpressed and 79 genes were underexpressed. Eight genes for which expression was at least twice as high or twice as low in iron-limited condition compared with iron-sufficient condition were chosen for RT-qPCR analysis to validate the RNA seq data. In general, most genes exhibited the same pattern of expression after 24 h of P. riograndensis growth under iron-limiting condition. Our results suggest that, during iron deficiency, bacteria express several genes related to nutrient uptake when they start to grow to obtain all of the molecules necessary for maintaining major cellular processes. However, once iron becomes highly limiting and is no longer able to sustain exponential growth, bacteria begin to express genes related to several processes, like sporulation and DNA protection, as a way of resisting this stress.
(Copyright © 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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