Effects of vitamin D3 and vitamin E on prednisolone-induced alterations of phagocyte function.
| Autor: | Shymanskyy IO; O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine. ishymansk@inbox.ru., Lisakovska OO, Mazanova AO, Riasniy VM, Veliky MM |
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| Jazyk: | angličtina |
| Zdroj: | European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2016 Apr; Vol. 20 (7), pp. 1379-83. |
| Abstrakt: | Objective: To evaluate the effectiveness of vitamin D3 and its combined action with vitamin E in the correction of the impairments of phagocyte function caused by chronic glucocorticoid administration. Materials and Methods: Phagocytic activity was assessed by the ability of peripheral blood neutrophils and monocytes to capture FITC-labeled Escherichia coli using flow cytometry. Metabolic activity of neutrophils was measured cytochemically as nitro blue tetrazolium (NBT) reduction test. Intracellular reactive oxygen species (ROS) were detected by 2',7'-dichlorofluorescein fluorescence. Results: Prednisolone administration (5 mg/kg b.w., 30 days) was accompanied by vitamin D3 deficiency and decompensation of phagocyte function associated with antimicrobial activity (decrease in NBT reduction rate, ROS formation and phagocytic activity). Vitamin D3 co-administration with prednisolone and, to a greater extent, its combination with α-tocopherol (100 IU and 0.5 mg/rat, 30 days respectively) partially restored phagocyte function. Conclusions: These data suggest that vitamin D3 and α-tocopherol can prevent immunosuppressive effects of prednisolone through elevating the efficacy of oxygen-dependent mechanisms of phagocytosis and increasing the functional activity of phagocytic cells. |
| Databáze: | MEDLINE |
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