Development and characterization of Rift Valley fever virus-like particles.

Autor: Li YT; College of Animal Science and Technology, Shihezi University, Shihezi, China.; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China.; College of Animal Science, Henan Institute of Science and Technology, Xinxiang, China., Wang CL; College of Animal Science and Technology, Shihezi University, Shihezi, China.; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Zheng XX; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Wang HL; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Zhao YK; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Gai WW; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China.; College of Veterinary Medicine, Jilin University, Changchun, China., Jin HL; Changchun SR Biological Technology Co., Ltd., Changchun, China., Gao YW; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Li N; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Yang ST; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China., Xia XZ; College of Animal Science and Technology, Shihezi University, Shihezi, China.; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China.
Jazyk: angličtina
Zdroj: Genetics and molecular research : GMR [Genet Mol Res] 2016 Mar 28; Vol. 15 (1). Date of Electronic Publication: 2016 Mar 28.
DOI: 10.4238/gmr.15017772
Abstrakt: Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and spread by arthropod vectors. RVF is currently prevalent in Africa and the Arabian Peninsula, and causes substantial economic losses. Furthermore, this disease poses a serious threat to animal and human health in regions worldwide, making it a serious public health concern. However, RVFV vaccines for human use are still unavailable, and hence there is an urgent need for novel efficient vaccines against RVFV. Vaccine preparation techniques have become a crucial factor in developing new vaccines. In the current study, the N and G protein genes of RVFV were inserted into the pFastBacDual baculovirus expression vector downstream of the pP10 and pPH promoters. The resultant recombinant vector, pFastBacDual-S-M, was transfected into Sf9 insect cells by lipofection. The recombinant baculovirus, named rBac-N-G, was retrieved and infected into Sf9 insect cells to generate RVFV virus-like particles (VLPs). Using polyclonal antibodies against RVFV proteins in immunofluorescence and western blot analyses, we positively identified the presence of the RVFV proteins in VLP preparations. Sucrose density gradient centrifugation and transmission electron microscopy revealed that the morphology of the RVFV VLPs was consistent with previous reports of RVFV virions. This study describes a technique for efficient production of RVFV VLPs, and has laid the foundation for future VLP-based RVFV vaccines.
Databáze: MEDLINE