Expression of 3β-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch.

Autor: Gultiken N; Department of Obstetric and Gynecology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey. Electronic address: vetnilgun@gmail.com., Yarim M; Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey., Yarim GF; Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey., Gacar A; Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey., Mason JI; MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2016 Jul 15; Vol. 86 (2), pp. 572-8. Date of Electronic Publication: 2016 Feb 17.
DOI: 10.1016/j.theriogenology.2016.02.006
Abstrakt: The purpose of this study was to compare the expression of 3β-hydroxystreroid dehydrogenase (3β-HSD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the control group. For immunostaining a rabbit polyclonal, one raised against recombinant human type 2 (adrenal/gonadal) 3β-HSD was used. Progesterone (P4) concentrations were not statistically different between the groups. Strongly stained large interstitial cell groups in the ovarian medulla were observed particularly in CEH-pyometra group although these cells in the control group were weakly or moderately stained and existed singly or paired. The expressions of 3β-HSD in luminal epithelium (42.40 ± 22.40% vs. 18.42 ± 13.15%, P < 0.05) and glandular epithelium (32.80 ± 27.05% vs. 2.94 ± 7.79%, P < 0.01) of endometrium were significantly higher in CEH-pyometra group than those in the control group. The expression of 3β-HSD in CL was higher (29.38 ± 9.58% vs. 22.94 ± 4.97%) in CEH-pyometra group than that of control group although the differences were not significant (P > 0.05). Similarly, the significant increase in the expression of 3β-HSD in ovarian interstitial cells (33.86 ± 29.44 vs. 1.13 ± 2.97, P < 0.05) was found in CEH-pyometra group compared to the control group. The study revealed that 3β-HSD expression in the endometrium of canine CEH-pyometra was significantly high.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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