5α-Dihydrotestosterone enhances wound healing in diabetic rats.

Autor: Gonçalves RV; Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil. Electronic address: reggiani.goncalves@ufv.br., Novaes RD; Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, Alfenas, MG, Brazil., Sarandy MM; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Damasceno EM; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., da Matta SL; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., de Gouveia NM; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlandia, MG, Brazil., Freitas MB; Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Espindola FS; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlandia, MG, Brazil.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2016 May 01; Vol. 152, pp. 67-75. Date of Electronic Publication: 2016 Mar 20.
DOI: 10.1016/j.lfs.2016.03.019
Abstrakt: Unlabelled: Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response.
Aims: Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats.
Main Methods: Rats (302.23±26.23g, n=48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics Committee. Diabetes was induced by intraperitoneal injection of streptozotocin (60mg/kg). Three skin wounds (12mm diameter) were created on the animals' back, which were randomized into 6 groups according to the application received: VT group: Vehicle (lanolin), SA group: 0.9% saline solution, NC group: Non-diabetic, CP group: positive control (silver sulfadiazine 0001%), T1 group: Testosterone (10%), T2 group: Testosterone (20%) emulsified in lanolin. The applications were made daily within 21days, and tissues from different wounds were removed every 7days.
Key Findings: Both groups treated with testosterone (T1 and T2) showed a significantly higher proportion of type I and type III collagen fibers. Superoxide dismutase levels were significantly higher on days 7 and 14 in testosterone treated groups. Protein carbonyls and MDA were lower in both groups.
Significance: We conclude that groups treated with 5α-DTH showed a better healing pattern with complete wound closure, and proved to have a positive effect on the morphology of the scar tissue as well as an antioxidant stimulating effect during secondhand intention skin wounds repair in diabetic rats.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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