Processed stigmas of Crocus sativus L. imaged by MALDI-based MS.

Autor: Pittenauer E; Institute of Chemical Technologies and Analytics, Vienna University of Technologies, Vienna, Austria., Rados E; Institute of Chemical Technologies and Analytics, Vienna University of Technologies, Vienna, Austria., Koulakiotis NS; Department of Pharmacology, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Tsarbopoulos A; Department of Pharmacology, Medical School, National and Kapodistrian University of Athens, Athens, Greece., Allmaier G; Institute of Chemical Technologies and Analytics, Vienna University of Technologies, Vienna, Austria.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2016 Jun; Vol. 16 (11-12), pp. 1726-30. Date of Electronic Publication: 2016 May 09.
DOI: 10.1002/pmic.201500534
Abstrakt: The processed, i.e. dried under certain conditions, stigmas of Crocus sativus L. are one of the most expensive plant parts used commercially. For the color, aroma and biological activity a very complex mixture of glycolipids termed crocins are responsible. Therefore studying structural composition and distribution in the commercial plant material is of great interest. We showed successfully the application of a MALDI-based mass spectrometric imaging (MSI) approach for stigmas towards different crocin species. MSI opens up the investigation of processed plant materials in various fields allowing studying the processing in detail as well as adulteration attempts (which are quite frequent due to the price of the material). Furthermore, we could demonstrate that a similar number of crocins present in stigmas could be detected by MALDI MSI compared to the classical approach of analyzing the solvent-extract of stigmas by MALDI-MS.
(© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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