The effects of 22°C and 4°C storage of platelets on vascular endothelial integrity and function.

Autor: Baimukanova G; Blood Systems Research Institute, University of California, San Francisco, California., Miyazawa B; Department of Surgery, University of California, San Francisco, California., Potter DR; Blood Systems Research Institute, University of California, San Francisco, California., Gibb SL; Blood Systems Research Institute, University of California, San Francisco, California., Keating S; Blood Systems Research Institute, University of California, San Francisco, California., Danesh A; Blood Systems Research Institute, University of California, San Francisco, California.; Department of Laboratory Medicine, University of California, San Francisco, California., Beyer A; Blood Systems Research Institute, University of California, San Francisco, California., Dayter Y; Blood Systems Research Institute, University of California, San Francisco, California., Bruhn R; Blood Systems Research Institute, University of California, San Francisco, California., Muench MO; Blood Systems Research Institute, University of California, San Francisco, California., Cap AP; Coagulation and Blood Research Program, US Army Institute of Surgical Research, JBSA-FT Sam Houston, Texas., Norris PJ; Blood Systems Research Institute, University of California, San Francisco, California.; Department of Laboratory Medicine, University of California, San Francisco, California., Spinella P; Department of Pediatrics, Washington University in St Louis, St Louis, Missouri., Cohen M; Department of Surgery, University of California, San Francisco, California., Pati S; Blood Systems Research Institute, University of California, San Francisco, California.; Department of Laboratory Medicine, University of California, San Francisco, California.
Jazyk: angličtina
Zdroj: Transfusion [Transfusion] 2016 Mar; Vol. 56 Suppl 1, pp. S52-64.
DOI: 10.1111/trf.13455
Abstrakt: Background: Although a majority of the studies conducted to date on platelet (PLT) storage have been focused on PLT hemostatic function, the effects of 4°C PLTs on regulation of endothelial barrier permeability are still not known. In this study, we compared the effects of room temperature (22°C) stored and (4°C) stored PLTs on the regulation of vascular endothelial cell (EC) permeability in vitro and in vivo.
Study Design and Methods: Day 1, Day 5, and Day 7 leukoreduced apheresis PLTs stored at 4 or 22°C were studied in vitro and in vivo. In vitro, PLT effects on EC permeability and barrier function, adhesion, and impedance aggregometry were investigated. In vivo, using a mouse model of vascular leak, attenuation of vascular leak and circulating PLT numbers were measured.
Results: Treatment of EC monolayers with Day 5 or Day 7 PLTs, stored at both 22°C and 4°C, resulted in similar decreases in EC permeability on average. However, analysis of individual samples revealed significant variation that was donor dependent. Additional in vitro measurements revealed a decrease in inflammatory mediators, nonspecific PLT-endothelial aggregation and attenuated loss of aggregation over time to TRAP, ASPI, ADP, and collagen with 4°C storage. In mice, while 22°C and 4°C PLTs both demonstrated significant protection against vascular endothelial growth factor A (VEGF-A)-induced vascular leak 22°C PLTs exhibited increased protection compared to 4°C PLTs. Systemic circulating levels of 4°C PLTs were decreased compared to 22°C PLTs.
Conclusions: In vitro, 4°C-stored PLTs exhibit a greater capacity to inhibit EC permeability than 22°C-stored PLTs. In vivo, 22°C PLTs provide superior control of vascular leak induced by VEGF-A. This discrepancy may be due to increased clearance of 4°C PLTs from the systemic circulation.
(© 2015 AABB.)
Databáze: MEDLINE
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