Simultaneous determination of tricarboxylic acid cycle metabolites by high-performance liquid chromatography with ultraviolet detection.

Autor: Shurubor YI; Brain and Mind Research Institute, Department of Neurology, Weill Cornell Medical College, New York, NY 10021, USA; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Cooper AJ; Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA. Electronic address: arthur_cooper@nymc.edu., Isakova EP; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Deryabina YI; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Beal MF; Brain and Mind Research Institute, Department of Neurology, Weill Cornell Medical College, New York, NY 10021, USA., Krasnikov BF; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia; Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2016 Jun 15; Vol. 503, pp. 8-10. Date of Electronic Publication: 2016 Mar 19.
DOI: 10.1016/j.ab.2016.03.004
Abstrakt: Here we describe a simple high-performance liquid chromatography (HPLC) procedure for the simultaneous detection and quantitation in standard solutions of 13 important metabolites of cellular energy metabolism, including 9 tricarboxylic acid (TCA) cycle components and 4 additional metabolites. The metabolites are detected by their absorbance at 210 nm. The procedure does not require prior derivatization, and an analysis can be carried out at ambient temperature within 15 min. The significance of the current work is that the current HPLC procedure should motivate the development of simplified TCA cycle enzyme assays, isotopomer analysis, and determination of selected TCA metabolite levels in plasma/tissues.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: