RNA sequencing reveals region-specific molecular mechanisms associated with epileptogenesis in a model of classical hippocampal sclerosis.

Autor: Vieira AS; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL., de Matos AH; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL., do Canto AM; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL., Rocha CS; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL., Carvalho BS; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL., Pascoal VD; Faculty of Basic Sciences, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil UFF., Norwood B; Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany., Bauer S; Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany., Rosenow F; Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany., Gilioli R; Laboratory of Animal Quality Control (CEMIB), Rua 05 de junho, s/n. Cidade Universitária 'Zeferino Vaz'. Distrito de Barão Geraldo, Campinas, SP, 13083-877, BRAZIL., Cendes F; Department of Neurology, University of Campinas - UNICAMP, Campinas, SP, Brazil., Lopes-Cendes I; Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2016 Mar 03; Vol. 6, pp. 22416. Date of Electronic Publication: 2016 Mar 03.
DOI: 10.1038/srep22416
Abstrakt: We report here the first complete transcriptome analysis of the dorsal (dDG) and ventral dentate gyrus (vDG) of a rat epilepsy model presenting a hippocampal lesion with a strict resemblance to classical hippocampal sclerosis (HS). We collected the dDG and vDG by laser microdissection 15 days after electrical stimulation and performed high-throughput RNA-sequencing. There were many differentially regulated genes, some of which were specific to either of the two sub-regions in stimulated animals. Gene ontology analysis indicated an enrichment of inflammation-related processes in both sub-regions and of axonal guidance and calcium signaling processes exclusively in the vDG. There was also a differential regulation of genes encoding molecules involved in synaptic function, neural electrical activity and neuropeptides in stimulated rats. The data presented here suggests, in the time point analyzed, a remarkable interaction among several molecular components which takes place in the damaged hippocampi. Furthermore, even though similar mechanisms may function in different regions of the DG, the molecular components involved seem to be region specific.
Databáze: MEDLINE
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