Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells.

Autor: Roberto MM; Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mmr@rc.unesp.br., Matsumoto ST; Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, UFES - Universidade Federal do Espírito Santo, Avenida Fernando Ferrari, 514, Goiabeiras, 29075-910 Vitória, ES, Brazil., Jamal CM; Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, UFES - Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, 29040-090 Vitória, ES, Brazil., Malaspina O; Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil., Marin-Morales MA; Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mamm@rc.unesp.br.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2016 Jun; Vol. 33, pp. 9-15. Date of Electronic Publication: 2016 Feb 16.
DOI: 10.1016/j.tiv.2016.02.005
Abstrakt: The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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