| Autor: |
Vallino Costassa E; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Fiorini M; Dipartimento di Scienze Neurologiche Biomediche e del Movimento, Universitá di Verona, Policlinico 'G.B. Rossi' Borgo Roma, Verona, Italy., Zanusso G; Dipartimento di Scienze Neurologiche Biomediche e del Movimento, Universitá di Verona, Policlinico 'G.B. Rossi' Borgo Roma, Verona, Italy., Peletto S; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Acutis P; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Baioni E; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Maurella C; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Tagliavini F; Istituto Neurologico Carlo Besta, Milano, Italy., Catania M; Istituto Neurologico Carlo Besta, Milano, Italy., Gallo M; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Faro ML; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Chieppa MN; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Meloni D; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., D'Angelo A; Dipartimento di Scienze Veterinarie, Sezione Clinica Medica, Universitá di Torino, Grugliasco (TO), Italy., Paciello O; Dipartimento di Patologia e Sanitá Animale, Universitá di Napoli Federico II, Napoli, Italy., Ghidoni R; Laboratorio Marcatori Molecolari, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli Brescia, Italy., Tonoli E; Laboratorio Marcatori Molecolari, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli Brescia, Italy., Casalone C; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy., Corona C; Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy. |
| Abstrakt: |
Amyloid-β (Aβ) deposits are seen in aged individuals of many mammalian species that possess the same aminoacid sequence as humans. This study describes Aβ deposition in 102 clinically characterized cattle brains from animals aged 0 to 20 years. Extracellular and intracellular Aβ deposition was detected with 4G8 antibody in the cortex, hippocampus, and cerebellum. X-34 staining failed to stain Aβ deposits, indicating the non β-pleated nature of these deposits. Western blot analysis and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry revealed in Tris, Triton, and formic acid fractions the presence of different Aβ peptides, characterized mainly by C-terminally truncated forms. Exploration of the genetic variability of APOE, PSEN1, and PSEN2 genes involved in Alzheimer's disease pathogenesis revealed several previously unreported polymorphisms. This study demonstrates certain similarities between Aβ deposition patterns exhibited in cattle brains and those in the human brain in early stages of aging. Furthermore, the identification of the same Aβ peptides reported in humans, but unable to form aggregates, supports the hypothesis that cattle may be protected against amyloid plaque formation. |
