Nitric Oxide, Ethylene, and Auxin Cross Talk Mediates Greening and Plastid Development in Deetiolating Tomato Seedlings.

Autor:	Melo NK; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Bianchetti RE; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Lira BS; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Oliveira PM; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Zuccarelli R; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Dias DL; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Demarco D; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Peres LE; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Rossi M; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.)., Freschi L; Department of Botany, University of São Paulo, Sao Paulo 05508-090, Brazil (N.K.G.M., R.E.B., B.S.L., P.M.R.O., R.Z., D.L.O.D., D.D., M.R., L.F.); andDepartment of Biological Sciences, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba 13418-900, Brazil (L.E.P.P.) freschi@usp.br.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2016 Apr; Vol. 170 (4), pp. 2278-94. Date of Electronic Publication: 2016 Feb 01.
DOI:	10.1104/pp.16.00023
Abstrakt:	The transition from etiolated to green seedlings involves the conversion of etioplasts into mature chloroplasts via a multifaceted, light-driven process comprising multiple, tightly coordinated signaling networks. Here, we demonstrate that light-induced greening and chloroplast differentiation in tomato (Solanum lycopersicum) seedlings are mediated by an intricate cross talk among phytochromes, nitric oxide (NO), ethylene, and auxins. Genetic and pharmacological evidence indicated that either endogenously produced or exogenously applied NO promotes seedling greening by repressing ethylene biosynthesis and inducing auxin accumulation in tomato cotyledons. Analysis performed in hormonal tomato mutants also demonstrated that NO production itself is negatively and positively regulated by ethylene and auxins, respectively. Representing a major biosynthetic source of NO in tomato cotyledons, nitrate reductase was shown to be under strict control of both phytochrome and hormonal signals. A close NO-phytochrome interaction was revealed by the almost complete recovery of the etiolated phenotype of red light-grown seedlings of the tomato phytochrome-deficient aurea mutant upon NO fumigation. In this mutant, NO supplementation induced cotyledon greening, chloroplast differentiation, and hormonal and gene expression alterations similar to those detected in light-exposed wild-type seedlings. NO negatively impacted the transcript accumulation of genes encoding phytochromes, photomorphogenesis-repressor factors, and plastid division proteins, revealing that this free radical can mimic transcriptional changes typically triggered by phytochrome-dependent light perception. Therefore, our data indicate that negative and positive regulatory feedback loops orchestrate ethylene-NO and auxin-NO interactions, respectively, during the conversion of colorless etiolated seedlings into green, photosynthetically competent young plants. (© 2016 American Society of Plant Biologists. All Rights Reserved.)
Databáze:	MEDLINE
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