Cytocompatibility and biocompatibility of nanostructured carbonated hydroxyapatite spheres for bone repair.

Autor:	Calasans-Maia MD; Departamento de Cirurgia Oral, Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Melo BR; Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Alves AT; Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Resende RF; Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Louro RS; Departamento de Cirurgia Oral, Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Sartoretto SC; Faculdade de Odontologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil., Granjeiro JM; Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, Rio de Janeiro, Brazil., Alves GG; Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niteroi, RJ, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2015 Nov-Dec; Vol. 23 (6), pp. 599-608.
DOI:	10.1590/1678-775720150122
Abstrakt:	Objective: The aim of this study was to investigate the in vitro and in vivo biological responses to nanostructured carbonated hydroxyapatite/calcium alginate (CHA) microspheres used for alveolar bone repair, compared to sintered hydroxyapatite (HA). Material and Methods: The maxillary central incisors of 45 Wistar rats were extracted, and the dental sockets were filled with HA, CHA, and blood clot (control group) (n=5/period/group). After 7, 21 and 42 days, the samples of bone with the biomaterials were obtained for histological and histomorphometric analysis, and the plasma levels of RANKL and OPG were determined via immunoassay. Statistical analysis was performed by Two-Way ANOVA with post-hoc Tukey test at 95% level of significance. Results: The CHA and HA microspheres were cytocompatible with both human and murine cells on an in vitro assay. Histological analysis showed the time-dependent increase of newly formed bone in control group characterized by an intense osteoblast activity. In HA and CHA groups, the presence of a slight granulation reaction around the spheres was observed after seven days, which was reduced by the 42nd day. A considerable amount of newly formed bone was observed surrounding the CHA spheres and the biomaterials particles at 42-day time point compared with HA. Histomorphometric analysis showed a significant increase of newly formed bone in CHA group compared with HA after 21 and 42 days from surgery, moreover, CHA showed almost 2-fold greater biosorption than HA at 42 days (two-way ANOVA, p<0.05) indicating greater biosorption. An increase in the RANKL/OPG ratio was observed in the CHA group on the 7th day. Conclusion: CHA spheres were osteoconductive and presented earlier biosorption, inducing early increases in the levels of proteins involved in resorption.
Databáze:	MEDLINE
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