| Autor: |
Jagannadh VK; Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012., Bhat BP; Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012., Nirupa Julius LA; Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012., Gorthi SS; Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012. saisiva.gorthi@iap.iisc.ernet.in.; Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012. saisiva.gorthi@iap.iisc.ernet.in. |
| Abstrakt: |
In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer. |
